The Effect of Bilirubin on Laboratory Investigations on Serum Creatinine: A Comparison Study Between Jaffe Reaction and Creatinase Enzymatic Method With Creatinine in Phosphate Buffered Saline Solution and Serum

Objective: To determine the creatinine concentration in phosphate buffered saline solution and serum with different bilirubin concentrationsusing Jaffe reaction Creatinase method.Methods: In Phase 1, concentrations dilution series were 0, 2, 3, 4, 5, 6, 7, 8, 9 mg/dL 9, 12, 15,18, 21, 24, 27, 30 mg/dL, respectively. Each was spiked eleven used 1. Phase2, values 0.51, 2.41 7.33 11 concentrations. The total bilirubin, by Jaffereaction method measured.Results: showed a significant underestimation up to of 2 at all concentrations.From 3 onwards, overestimation observed high gave nosignificant 0.51 creatinine. But from bilirubinconcentration 7.18 mg/dL. creatinine, methods underestimations frombilirubin 9.05 5.64 given levels 3.6 8.18 methods, respectively.Conclusion: normal moderately is more accurate than allcreatinine used. At which increased withbilirubin concentration.